منابع مشابه
Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase
BACKGROUND The beta-galactosidase from Kluyveromyces lactis is a protein of outstanding biotechnological interest in the food industry and milk whey reutilization. However, due to its intracellular nature, its industrial production is limited by the high cost associated to extraction and downstream processing. The yeast-system is an attractive method for producing many heterologous proteins. Th...
متن کاملOptimization of β-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
This paper investigates the production and optimization of β-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four ferm...
متن کاملα-amylase and β-galactosidase production on Potato starch waste by Lactococcus lactis subsp lactis isolated from pickled yam
Potato starch waste, a chips industry effluent, was used for the production of industrially important enzymes by an amylolytic strain of lactic acid bacteria, isolated from pickled yam & identified as Lactococcus lactis. The strain was observed to co-produce α-amylase and β-galactosidase. Potato starch waste was efficiently utilized (91.6%) along with high co-production of α-amylase and β-galac...
متن کاملPyruvate kinase of Streptococcus lactis.
The kinetic properties of pyruvate kinase (ATP:pyruvate-phosphotransferase, EC 2.7.1.40) from Streptococcus lactis have been investigated. Positive homotropic kinetics were observed with phosphoenolpyruvate and adenosine 5'-diphosphate, resulting in a sigmoid relationship between reaction velocity and substrate concentrations. This relationship was abolished with an excess of the heterotropic e...
متن کاملExtracellular Proteinase of Streptococcus Lactis.
Williamson, W. T. (North Carolina State of the University of North Carolina, Raleigh), S. B. Tove, and M. L. Speck. Extracellular proteinase of Streptococcus lactis. J. Bacteriol. 87:49-53. 1964.-Streptococcus lactis was shown to produce an extracellular proteolytic enzyme(s). A 120-fold purification of the proteinase was obtained from the cell-free culture medium by ammonium sulfate fractionat...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1965
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.89.4.937-942.1965